labeling technique中文什么意思

發(fā)音:

用"labeling technique"造句

標(biāo)記原子

label: n. 1.紙條，貼條，標(biāo)簽，簽條。 2.稱號(hào)，綽號(hào)。 3 ...
technique: n. 1.(專門)技術(shù)；(藝術(shù)上的)技巧，技能。 2.手 ...
affinity labeling technique: 親和標(biāo)記技術(shù),親和示蹤技術(shù)
cell labeling technique: 細(xì)胞標(biāo)記技術(shù)
deuterium labeling technique: 氘標(biāo)記術(shù)

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By using the gfp - labeling technique , we have found that gfp - cam colocalized with all the characterized structures formed during cytokinesis
通過采用gfp標(biāo)記技術(shù)，我們觀察到gfp - cam與胞質(zhì)分裂期細(xì)胞內(nèi)形成的特征結(jié)構(gòu)存在共分布。
But there are still no reports about the relationships of dnpi - like and gabaergic immunoreactive ( gad - li ) terminals with pag - like immunoreactive ( pag - li ) neurons in " zone - shaped area " . to answer these questions , we observed systemically the synaptic connections among the pv - like immunoreactive neurons , fibers and terminals and the connections between dnpi - like , gabaergic terminals and pag - li neurons using the methods of electron microscopic imrnunohistochemistry , triple - immunofluorescence histochemistry and retrograde tracing method combined with pre - embeded immunoelectron microscopic double - labeled technique
但是目前對(duì)新發(fā)現(xiàn)的囊泡膜mu轉(zhuǎn)運(yùn)體一dnn樣陽性終末與帶狀區(qū)內(nèi)pag樣陽性神經(jīng)元之間ej關(guān)系，以及谷氨酸脫發(fā)酶（ gad ，是gaba能神經(jīng)元和終末的特異性標(biāo)識(shí)物）是否參與其調(diào)控作用，尚缺乏系統(tǒng)的形態(tài)學(xué)資料。
The present results indicated that the paraventricular nucleus of the hypothalamus and the supraoptic nucleus might have important roles in neuroimmunomodulation . 2 . following lps or seb was administered intraperitoneally , the expression of pcna of splenic cells and il - 1 receptor type i in pvn and son were observed by using immunocytochemistry in the mice . double fluorescent labeling technique was used to determine the relationship of il - 1 receptor type i co - expressions with arginine vasopressin or oxytocin
二、小鼠腹腔內(nèi)給予細(xì)菌內(nèi)毒素lps或腸毒素seb ，用免疫組織化學(xué)方法觀察了脾臟核增殖抗體及下丘腦室旁核和視上核中1型il 1受體的表達(dá)，并采用雙標(biāo)記技術(shù)觀察了1型il刁受體陽性神經(jīng)元和加壓素及催產(chǎn)素表達(dá)的關(guān)系。
The amplification system was optimized so that the pcr with different primers can be carried out under the same condition . the pcr products were sequenced using sanger ' s terminator and fluorescent label techniques . sequences were analyzed and compared base on sequencing analysis3 . 4 and seq / ede software
方法根據(jù)mtdna控制區(qū)及其周圍區(qū)域的序列，設(shè)計(jì)多對(duì)引物，探索優(yōu)化擴(kuò)增體系，使擴(kuò)增條件能夠同時(shí)滿足多對(duì)引物的需要，用sanger末端終止法及熒光標(biāo)記技術(shù)對(duì)樣本進(jìn)行dna測序， sequencinganalysis3 . 4和seq ede軟件進(jìn)行序列分析和比對(duì)。
The characteristics of this method are : a , directly counting cell number without the influence of the metabolic state of the cells ; b , discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c , less treatment step , and free - radioactivity ; d , high sensitivity and reliability . 2 , using the above assay , immunofluorescent labeled technique , and flow cytometry , the pbmc proliferation , apoptosis , necrosis , cell cycle , activation , cytokines and membrane marker were detected . the results showed that the number of pbmc reduced , but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle , but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased
結(jié)合以上創(chuàng)建的方法和免疫熒光流式細(xì)胞術(shù)，用k562細(xì)胞株可溶性分泌物(上清)對(duì)外周血單個(gè)核細(xì)胞( pbmc )進(jìn)行培養(yǎng)以模擬體內(nèi)微環(huán)境，然后分別從細(xì)胞增殖、凋亡、壞死、細(xì)胞周期、活性、細(xì)胞因子和表面抗原表達(dá)等方面進(jìn)行研究，結(jié)果發(fā)現(xiàn)用腫瘤上清培養(yǎng)的pbmc細(xì)胞數(shù)量下降明顯，但同時(shí)對(duì)其有激活作用，且呈劑量依賴性；細(xì)胞數(shù)的下降主要是由細(xì)胞壞死和凋亡引起的，腫瘤上清對(duì)細(xì)胞周期沒有阻斷作用，反而略有促進(jìn)作用； t細(xì)胞亞群比例增加，并促進(jìn)表達(dá)th1 、 th2細(xì)胞因子。